Figure 2.
Ribavirin inhibits Akt activation mediated by eIF4E overexpression. (A) Western blots analysis of murine fibroblasts overexpressing eIF4E treated with 1µM ribavirin or tiazofurin for 48hrs. Detected proteins are as indicated, with β-actin shown as a loading control. (B) RNA quantification from qPCR experiments from murine fibroblasts showing the relative fold difference for NBS1 mRNA cytoplasmic/nuclear (C/N) ratio. (C) Ribavirin inhibits the expression of a lacZ chimeric model construct, which contains a 4E-SE, but not the lacZ control. LacZ was detected using an antibody against the Xpress tag. α-tubulin is shown as a loading control. (D) RNA quantification from qPCR experiments showing the relative fold difference of lacZ mRNA C/N ratio. C/N ratios ± SD were normalized to vector control, arbitrarily set to 1. Averaged values were normalized to GAPDH levels. Fractionation controls (U6 snRNA – nuclear, tRNAlys – cytoplasmic) and total NBS1 and lacZ mRNA levels are shown in Supplementary Figure S1.