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. 1997 Dec;8(12):2659–2676. doi: 10.1091/mbc.8.12.2659

Figure 10.

Figure 10

Multicopy suppression of sft1–1 and vti1 ts growth defect. (A) Vti1p overexpression suppresses the growth defect of the sft1–1. Growth at 35°C on YPD plates of the sft1–1 strain carrying multicopy plasmids bearing VTI1, SFT1, or no insert as indicated. (B) Growth at (37.5°C) on YPD plates of the indicated mutants carrying 2μ multicopy plasmids bearing the indicated genes. +++, wild-type growth; ++, good growth; +, poor growth; NS, no suppression. (C) Human Vti1p homologue (hVti1-rp1) expression suppresses the growth defect of the vti1–25 mutant allele. Growth at 37°C on YPGal plates of the vti1–25 strain carrying multicopy PGAL plasmids bearing VTI1, hVti1-rp1, mVti1-rp2, or no insert as indicated. YKT6 was not under PGAL control.