Table 2.
S. cerevisiae strains used in this work
| Strain | Genotype | Source |
|---|---|---|
| RSY255 | MATα ura3-52 leu2-3,-112 | R. Schekman |
| RSY271 | MATα sec18-1 ura3-52 his4-612 | R. Schekman |
| RSY272 | MATa sec18-1 ura3-52 his4-612 | R. Schekman |
| GWY30 | MATa/α ura3-52/ura3-52 leu2Δ1/leu2Δ1 trp1Δ63/trp1Δ63 | This laboratory |
| GWY147 | MATα ura3-52 his3Δ200 leu2-3,-112 trp1Δ901 sft1Δ::LEU2 psft1-1 (CEN, HIS3) | H. Pelham |
| GWY150 | MATa/α ura3-52/ura3-52 leu2Δ1/leu2Δ1 trp1Δ63/trp1Δ63 vti1Δ::LEU2/VTI1 | This study |
| GWY151 | MATa ura3-52 leu2Δ1 trp1Δ63 vti1Δ::LEU2 pPI2 (2μ, URA3, VTI1) | This study |
| GWY152 | MATa ura3-52 leu2Δ1 trp1Δ63 vti1Δ::LEU2 pPI6 (2μ, URA3, PGAL-GST-VTI1 | This study |
| GWY153 | MATa ura3-52 leu2Δ1 trp1Δ63 vti1Δ::LEU2 pPI7 (CEN, TRP1, PCUP-HA-VTI1) | This study |
| GWY154 | MATa ura3-52 leu2Δ1 vti1Δ::LEU2 PCUP-HA-VTI1 | This study |
| GWY155 | MATα ura3-52 leu2Δ1 trp1Δ63 vti1Δ::LEU2 pPI9(CEN, TRP, PGAL-HA-VTI1) | This study |
| GWY156 | MATα ura3-52 trp1Δ63 vti1Δ::LEU2 sec18-1 pPI6 (2μ, URA3, PGAL-GST-VTI1) | This study |
| GWY157 | MATα ura3-52 vti1Δ::LEU2 sec18-1 PCUP-HA-VTI1 | This study |
| GWY158 | MATa ura3-52 leu2Δ1 trp1Δ63 vti1Δ::LEU2 pPIts3 (CEN, TRP1, PCUP-HA-vti1-21) | This study |
| GWY159 | MATa ura3-52 leu2Δ1 trp1Δ63 vti1Δ::LEU2 pPIts4 (CEN, TRP1, PCUP-HA-vti1-22) | This study |
| GWY160 | MATa ura3-52 leu2Δ1 trp1Δ63 vti1Δ::LEU2 pPIts6 (CEN, TRP1, PCUP-HA-vti1-23) | This study |
| GWY161 | MATa ura3-52 leu2Δ1 trp1Δ63 vti1Δ::LEU2 pPIts7 (CEN, TRP1, PCUP-HA-vti1-24) | This study |
| GWY162 | MATa ura3-52 leu2Δ1 trp1Δ63 vti1Δ::LEU2 pPIts16 (CEN, TRP1, PCUP-HA-vti1-25) | This study |