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Overexpression of Bim1p causes arrest at the large budded stage with undivided nuclei. GAL1 promoter-driven overexpression of Bim1p in DBY6654 transformed with pRB2652 was induced by growth in 2% galactose for 10.5 h. Nomarski images (A and C) are shown next to combined images of DAPI-stained DNA and antitubulin-stained microtubules (B and D). Bar, 4 μm.
