Table 5.
Temperature (°C) | BIM1 | bim1Δ | BIM1 | bim1Δ | BIM1 | bim1Δ | BIM1 | bim1Δ | BIM1 | bim1Δ |
---|---|---|---|---|---|---|---|---|---|---|
12 | 85 | 42 | 2 | 15 | 0 | 13 | 12 | 15 | 1 | 15 |
30 | 85 | 35 | 0 | 14 | 0 | 1 | 14 | 33 | 1 | 17 |
38 | 73 | 20 | 2 | 6 | 5 | 1 | 15 | 44 | 4 | 29 |
Strains DBY6654 (BIM1), DBY7303 (bim1Δ), and DBY7305 (bim1Δ) were grown at 30°C in YPD to exponential phase, shifted for two generation times to 12°C (24 h) or to 38°C (3.5 h), and stained with DAPI. Numbers shown are the percentage of large budded cells (always at least 100) counted. Data from two bim1Δ strains (DBY7303 and DBY7305) are similar and presented as an average.