Table 8.

Differential genetic interactions of tub1 alleles with bim1Δ compared to differential two-hybrid interactions with BIM1 and BIK1^a

Allele	Phenotype	Genetic interaction with bim1Δ	2-Hybrid interaction with BIM1p	2-Hybrid interaction with Bik1p
TUB1	WT		+	+
tub1-801	BenSS, cs, ts	SL	+	+
tub1-802	RL	ND	+/−	+
tub1-803	BenSS	SP	+	+
tub1-804	BenSS	None	+	+
tub1-805	BenSS	None	+	+
tub1-806	BenR	SP	+	+
tub1-807	BenSS	None	+	+
tub1-808	RL	ND	−	+
tub1-809	BenSS, slow	None	−	+
tub1-810	BenSS	None	+	+
tub1-811	WT	None	+	+
tub1-812	BenSS	SP	+	+
tub1-813	BenSS, cs, ts	SP	−	+
tub1-814	BenSS, slow	SS	−	−
tub1-815	WT	None	+	+
tub1-816	BenSS, cs	SP	+	+
tub1-817	BenSS	SP	+	+
tub1-818	BenSS, cs	SP	−	+
tub1-819	BenSS	None	+	+
tub1-820	DL	ND	Lethal	ND
tub1-821	BenSS	SP	−	+
tub1-822	BenSS, cs, ts	SP	+	+
tub1-853	BenSS, cs, ts	SL	+	+
tub1-823	RL	ND	−	−
tub1-824	BenSS, cs	SP	+	+
tub1-825	BenR	SP	+	+
tub1-826	BenR	SP	+	+
tub1-827	BenSS cs, ts	None	+	+
tub1-828	DL	ND	Lethal	ND
tub1-829	BenSS, cs, ts	SS	+	+
tub1-830	BenSS	SP	+	+
tub1-831	BenSS, cs	SL	−	+
tub1-832	BenR	SP	+	+
tub1-833	BenSS	None	+	+
tub1-834	BenSS, cs, ts	SS	−	−
tub1-835	WT	None	+	+
tub1-836	BenSS, cs, ts	SS	+	+
tub1-837	BenSS	None	+	+
tub1-838	BenSS	SP	+	+
tub1-839	BenR	None	+	+
tub1-841	BenR	SS	+	+
tub1-842	BenSS, cs, ts	SS	−	+
tub1-845	BenSS, cs, ts	SP	−	−
tub1-846	BenSS, cs, ts	SL	−	+
tub1-847	BenSS, cs, ts	SS	−	+
tub1-848	BenSS, cs, ts	SL	−	+/−
tub1-849	BenSS, cs, ts	SS	−	+
tub1-850	BenSS	SP	+	+
tub1-851	BenSS	None	+	+
tub1-852	BenSS	SP	+	+

^atub1 alleles are listed in 5′ to 3′ order in the first column. The phenotype resulting from each tub1 allele alone is shown in the second column (WT, wild-type; DL, dominant lethal; RL, recessive lethal; BenSS, benomyl-supersensitive; cs, cold-sensitive; ts, heat-sensitive; slow, poor growth at all temperatures). The third column shows the effects of combining each tub1 allele with bim1Δ (none, no synthetic phenotype; SP, synthetic phenotype; SS, synthetic “sickness”; SL, synthetic lethality; ND, not done). Columns 4 and 5 show the results of testing two-hybrid interactions of the tub1 alleles with BIM1 and BIK1, respectively. Strains obtained by cotransforming Y190 with various combinations of bait plasmids and library isolates containing BIK1 or BIM1 fused to the GAL4 activation domain were spotted on SD + 10 μg/ml adenine and 50 mM 3-amino-1,2,4-triazole. Interaction was scored as +, robust growth; +/−, intermediate growth; or −, poor or no growth.