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. Author manuscript; available in PMC: 2008 Oct 27.
Published in final edited form as: Biochemistry. 2007 Feb 14;46(11):3246–3254. doi: 10.1021/bi602499t

Figure 6.

Figure 6

Reduction of ERV2p in the presence of zinc ions. Erv2p (23 µM in Tris buffer, pH 7.5; containing 50 µM Zn2+; curve 1) was titrated anaerobically with 2, 3, 4, 6 and 8 electron-equivalents of dithionite/mol of flavin (curves 2–6 respectively). Intermediate spectra are omitted for clarity. The inset plots the absorbance changes at 318 nm (triangles), 450 nm (squares) and 560 nm (diamonds) respectively.