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. 2008 Nov 7;4(11):e1000246. doi: 10.1371/journal.pgen.1000246

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Bannasch et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) The electropherograms for the SLC2A9 sequence from a non-Dalmatian dog (+/+), heterozygous backcross dog (+/huu), and a Dalmatian dog (huu/huu) are shown. The nucleotide sequence is given, with the amino acid sequence presented below. The box shows the location of the mutation. (B) Protein sequence alignment of SLC2A9 around the exon 5 missense mutation from Dalmatians. The altered SLC2A9 amino acid is indicated with a vertical arrow. The horizontal line indicates the fifth transmembrane domain of the protein. The C. familiaris sequence is shown for Boxer (B) and Dalmatian (D).