Figure 5.

TGase activity of HSCs does not prevent its proliferation. Hpt cells were cultured on coverslips, and maintained in six well plates. The cells were labeled with 1 mM 5-(biotinamindo)-pentylamine (BPNH₂; Molecular Probes, Pierce) as a substrate for TGase and 10 μM BrdU (Sigma) for 2 or 16 hours. The in situ TGase activity was stained by streptavidin-Cy5, BrdU incorporation assay was measured by staining with anti-BrdU-FITC. A-D. Control cells incubated without BrdU and BPNH_2.E-H. Cells incubated with BrdU and BPNH₂ for 2 hours. I-L. Cells incubated with BrdU and BPNH₂ for 16 hours. M-P. Cell surface TGase activity detected in non-permeabilized cells after incubation with BPNH₂ for 16 hours.