Figure 10.

Two-hybrid analysis of Pex17p and Pex19p. The indicated Pex17p and Pex19p hybrid protein constructs were tested for trans-activation of the HIS3 gene, resulting in growth on media lacking histidine, or LacZ, resulting in the production of β-galactosidase assayed as described in MATERIALS AND METHODS. Numbers refer to amino acids from Pex19p (A) or Pex17p (B). pAD, transcriptional activation domain fusion constructs; pBD, DNA-binding domain fusion constructs; C, empty DNA-binding domain plasmid (pKNSD55).