Figure 11.

Cross-linking and coimmunoprecipitation of Pex17HAp with the PTS–receptor docking complex and Pex19p. Immunoprecipitations from cross-linked (+) or non–cross-linked (−) extracts of the Pex17HAp-expressing strain (SWS17HA) were analyzed by immunoblotting. (A) Pex19p, Pex5p, and Pex7p were immunoprecipitated and immunoblotted with anti-HA. (B) Pex17HAp and Pex14p were immunoprecipitated and immunoblotted with anti-Pex3p. (C) Pex14p was immunoprecipitated and immunoblotted with anti-Pex19p. (D) Pex19p, Pex5p, and Pex7p were immunoprecipitated and immunoblotted with anti-Pex3p. Whole-cell lysates (wc) were loaded (0.033 A₆₀₀) as a control for immunoblotting.