Figure 6.

Mislocalization of peroxisomal proteins by pex17Δ mutants. (A) Oleate-grown spheroplasts of wild-type and pex17Δ cells (SMD1163 and SWS17D) were lysed and subjected to sequential differential centrifugation. Equivalent amounts of the PNS, 27,000 × g supernatant (S27), 27,000 × g pellet (P27), 100,000 × g supernatant (S100), and 100,000 × g pellet (P100) were resolved by SDS-PAGE, transferred to nitrocellulose, and probed with the indicated antibodies. (B) The PNS from oleate-grown cells was adjusted to 65% sucrose, layered with 50 and 35% sucrose, and centrifuged to allow floatation of membranes into the lighter fractions as described in MATERIALS AND METHODS. P, pelleted material at the bottom of the tube. Arrows point to the normal, full-length catalase and Pex3p.