Skip to main content
. 1999 Dec;10(12):4005–4019. doi: 10.1091/mbc.10.12.4005

Figure 7.

Figure 7

Subcellular localization of Pex17HAp. (A) Oleate-grown spheroplasts of pex17Δ cells expressing Pex17HAp (SWS17HA) were lysed and subjected to sequential differential centrifugation. Equivalent amounts of the PNS, 27,000 × g supernatant (S27), 27,000 × g pellet (P27), 100,000 × g supernatant (S100), and 100,000 × g pellet (P100) were resolved by SDS-PAGE, transferred to nitrocellulose, and probed with the indicated antibodies. (B) Methanol-grown spheroplasts of pex17Δ cells expressing Pex17HAp (SWS17HA) were indirectly labeled using anti-Pex3p or anti-HA antibodies as described in MATERIALS AND METHODS and visualized by fluorescence microscopy (Pex3p and Pex17HAp) and Nomarski optics.