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. Author manuscript; available in PMC: 2009 Jun 27.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Apr 25;371(2):333–338. doi: 10.1016/j.bbrc.2008.04.084

Figure 1. LYRIC/AEG-1 associates with BCCIP.

Figure 1

(a) 293T cells were co-transfected with plasmids encoding full-length FLAG-tagged LYRIC/AEG-1 with empty HA-vector (lanes1&4), HA-BCCIPc (lanes 2&5) or HA-BCCIPα (lanes 3&6). Expression was verified by Western blotting of cell lysates with anti-FLAG and anti-HA (lanes 1–3). FLAG-LYRIC was immunoprecipitated using anti-FLAG agarose (lanes 4–6, upper), and both isoforms of BCCIP co-precipitated (lanes 4–6, lower). (b) HA-BCCIPα (+) or empty vector (−) were transfected into 293T and endogenous LYRIC was IP’d using PAb5393. IP with pre-immune rabbit serum (IgG) was the negative control. BCCIPα co-precipitated with LYRIC (upper), and precipitation of LYRIC was confirmed by blotting with PAb5393 (lower). (c) HA-BCCIPα was transfected into 293T and IP’d with anti-HA agarose. Endogenous LYRIC co-precipitated and was detected with PAb5393.