Figure 2. NH2-terminal region of LYRIC is required for association with BCCIPα.
(a) FLAG-tagged deletion constructs, indicating the region expressed, drawn to scale with full-length LYRIC (RNF). (b) 293T cells were transfected with BCCIPα and each of the LYRIC constructs. Protein expression was verified by Western blotting of total cell lysates (left). BCCIPα was precipitated with anti-HA agarose, and only constructs Δ238 and Δ463, and full length LYRIC co-precipitated.