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. 2008 Nov 5;3(11):e3632. doi: 10.1371/journal.pone.0003632

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Seoane et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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a, Growth curve analysis of early-passage cRb ^loxP/loxP conditional astrocytes co-infected with PIG/pBABE (vector), PIG/pBABE-HRas^V12, PIG-Cre/pBABE and PIG-Cre/pBABE-HRas^V12 retroviral vectors. After infection, cells were plated in triplicate and the cells were fixed on the indicated days for subsequent staining with crystal violet. Each time point represents the mean±s.d. of total cumulative cell number from at least three independent experiments. b, Measurement of the proliferation of astrocytes by BrdU incorporation assay (panel c). c, Cells were labeled with BrdU for 5 h on day 5 after puromycin selection. d, Senescence assays in cRb^loxP/loxP and cRb^−/−. The y-axis represents the percentage of SA-β-galactosidase-positive cells (mean and s.d.) from at least three independent experiments. e, A representative result of three independent experiments is shown. Photographs are at the same magnification.