Figure 2. The effect of Rest ablation on the expression of stage-specific neural markers.
The ability of control (REST-100) and Rest mutant ES cells (REST/KD-50 and REST-null) to generate the different neural cell populations was assessed by real time-PCR using the following markers: NSCs Pax6, Msi1 and Nestin (A–C); NPCs Mash1 and Ngn1 (D–E); early neurons Tubb3 (F) and mature neurons, Scn2a and L1cam (G–H). Data are represented as mean±SEM. *P<0.05 and **P<0.01, significantly different from REST-100 and REST/KD-50.