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. 2008 Nov 6;3(11):e3656. doi: 10.1371/journal.pone.0003656

Figure 5. Laminins rescue the phenotypic effects of Rest ablation on the neural differentiation of ES cells.

Figure 5

Control (REST-100) and 4 Rest mutants ES cells were grown under neural-differentiating conditions on laminin coated glass coverslips. After 4 days of differentiation some cultures were fixed and labelled with the NSC markers Nestin (red) and Sox1 (green) (A–E). The results show that laminins restored the ability to generate Nestin+ cells in Rest mutants (C–E, compared with Fig. 3C–3E). Sister cultures were allowed to differentiate for 14 days to analyse neuronal differentiation using NeuN (red) and Map2 (green) (F–J and in higher power K–O). Laminins rescued the neuronal phenotypes derived from REST-null ES cells, which included elaboration processes, neurite outgrowth and migration (see arrows in M and N) similar to that seen in neurons derived from control ES cells. Nuclei were stained with DAPI (blue) in all. Scale bars: 20 µm (A to E and K to O) and 100 µm (F to J).