Figure 2.

Immunoperoxidase EM analysis of cells treated with BEL and nocodazole. Cells were either untreated (A), treated with nocodazole (6 μg/ml) for 2 h (B), or pretreated with 5 μM BEL followed by nocodazole (6 μg/ml) in the continued presence of BEL for 2 h (C). Cells were then fixed and incubated with rabbit anti-ManII antibodies, followed by sheep anti-rabbit-HRP conjugates, and processed for diaminobenzidine cytochemistry as described previously (Brown and Farquhar, 1989) (see MATERIALS AND METHODS).