Figure 4.

Dose response of ONO-RS-082 inhibition of nocodazole-induced ministack formation. Cells were pretreated with various concentrations of ONO-RS-082 followed by nocodazole for 2 h before fixing and processing for immunofluorescence microscopy, using a polyclonal antibody against ManII to visualize the Golgi. The percentage of cells with ManII in the juxtanuclear region, ER (as determined by nuclear envelope staining), or ministacks was quantified, with each point representing the average of two experiments with 200 cells counted in each experiment.