Figure 5.

Colocalization of ManII with the resident ER enzyme PDI in cells treated with 5 μM ONO-RS-082 and nocodazole. Clone 9 cells were untreated (A and B) or treated with 5 μM ONO-RS-082 (C and D) at 4°C for 20 min before shifting to 37°C in the continued presence of antagonist plus 6 μg/ml nocodazole for 2 h. All conditions included 2 μg/ml cycloheximide. Cells were processed for double-label immunofluorescence microscopy using a polyclonal antibody against ManII (A and C) and a monoclonal antibody against PDI (B and D).