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Kinetic analysis of ACA-induced redistribution of ManII to the ER. Cells were incubated in the presence or absence of ACA (25 μM) for up to 4 h as indicated on the micrographs and then were fixed and stained for ManII immunofluorescence. In all cases, cells were pretreated with 50 μg/ml cycloheximide for 15 min and then incubated in its continuous presence after addition of ACA. n, nucleus.
