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. 1999 Dec;10(12):4021–4032. doi: 10.1091/mbc.10.12.4021

Figure 9.

Figure 9

ONO-RS-082, but not BEL, inhibits ER-to-Golgi transport of the VSVG protein. Clone 9 cells were infected with VSVts045 at 40°C and kept at the restrictive temperature (40°C) for 3 h to accumulate the VSVG protein in the ER (see MATERIALS AND METHODS). Transport of the VSVG protein was followed by immunofluorescence microscopy using a monoclonal antibody against the VSVG protein (A, C, E, and G) and a polyclonal antibody against ManII (B, D, F, and H). Cells were kept at 40°C (A and B) or shifted to the permissive temperature 32°C for 30 min in the absence (C and D) or presence of ONO-RS-082 (E and F) or BEL (G and H). In J, the percentage of cells with the VSVG protein in the Golgi at 32°C in the presence of ONO-RS-082 and BEL was determined. Colocalization of the VSVG protein and ManII was counted as transport of the VSVG protein to the Golgi. Each column represents the average of two experiments with 100 cells counted in each experiment.