Table I.
Primer and probe sequences for CNR1 exon 3 transript inititation 5′ flanking SNPs with SNP postion relative to exon 3 alternate transcript initiation site.
| SNP (Position) | Primers | MGB-Probe | [Probe] |
|---|---|---|---|
| rs6928499 (−407) | F-CCTAAATCGCAGAACTGATCTGAA
R-GCAAAGAGCCATAATACTAAGTAATGATAA |
VIC-ATGTAAAACATAGTGCCTGAC
FAM-TAAAACATACTGCCTGAC |
160 nM VIC
120 nM FAM |
| rs806379 (−385) | F-AATGCCTAAATCGCAGAACTGATCT
R-AATAATACCCATTGAAGACTTACTTTGTGTCA |
VIC-ACATGCATTTAATATCATC
FAM-TAACATGCATTTAATTTCATC |
100 nM each |
| rs1535255 (−326) | F-CTTGGGCAATCAGTCTTTCTAAGC
R-AGATCAGTTCTGCGATTTAGGCATT |
VIC-CTCATCCCCCTTTTAC
FAM-CATCCCCATTTTAC |
100 nM each |
| rs2023239 (+400) | F-GAGTTGAAAGGCAAAAGCTAGGTTT
R-GGGACACAGAAGACAGTCACAATAT |
VIC-CTGTTCCTTACGTGGTCC
FAM-TGTTCCTTACATGGTCC |
140 nM VIC
100 nM FAM |