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. 2008 Oct 17;5(6):295–302. doi: 10.7150/ijms.5.295

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Cocaine-induced nucleosomal DNA fragmentation on agarose gels in fetal rat brain. Cocaine was administered subcutaneously to the pregnant rats for 7 days as described in Methods. Cocaine-induced nucleosomal DNA fragmentation in fetal brain was separated in 1.8% agarose gels. The apoptotic DNA ladders shown were in the brain treated with 30 mg/kg/day (lane 3) and 60 mg/kg/day (lane 4), but not in the control brain (lane 2). DNA markers, _ØX174 DNA fragments cut by HaeIII as size marker are shown in lane 1. The same results were obtained from five additional separate experiments.