Figure 1.

Cocultivation of primary rat hepatocytes with WT-CHO or T-cad+ CHO cells on collagen-coated substrates. A) Morphology (representative day 15) of cocultures and pure hepatocyte cultures (hepatocytes indicated by arrows). Scale bars = 100 μm. B) Hepatocyte functions (representative day 10) in pure cultures and cocultures. Similar trends were seen for at least 2 wk of culture in 3 independent biological repeat experiments. *P < 0.05; 2-tailed, unpaired Student’s t test. Error bars = sem (n=3).