Figure 1.

Identification of SNAK, a SNARE kinase. (A) Domain structure of SNAK. The presence of the coiled-coil domain within SNAK was identified by the COILS algorithm (Lupas, 1996), and the presence of the kinase domain and the specified subdomains was determined by aligning the primary sequence of SNAK with that of other serine/threonine kinases. (B) Northern blot analysis of SNAK expression. A human tissue Northern blot containing poly(A)⁺ RNA from heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas was probed with a SNAK probe under high-stringency hybridization conditions. A single 4.4-kilobase transcript was detected in all tissues examined in this and other Northern blots.