Figure 7.
SNAK kinase activity is required to promote SNAP-23 assembly with syntaxin. HeLa cells expressing SNAP-23 and syntaxin 4 in the presence of wild-type SNAK (left panel) or SNAP-23 and syntaxin 4 in the presence of a catalytically inactive SNAK mutant (right panel) were labeled with [35S]methionine for 15 min and chased for various times. After cell lysis in Triton X-100, lysates were immunoprecipitated with the use of control (C) or anti-syntaxin 4 serum and analyzed by SDS-PAGE and fluorography.