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. 1999 Dec;10(12):4043–4057. doi: 10.1091/mbc.10.12.4043

Figure 2.

Figure 2

Relocalization of MCM proteins in mcm mutant is crm1 dependent. (A) mcm3-HA cells fail to form colonies at 17°C. Assay of colony formation of wild-type and mcm3-HA cells at 32°C for 4 d or 17°C for 13 d. (B and C) MCM protein localization in mcm3-HA. Cells were incubated at 32°C or the restrictive temperature (17°C) for 6 h before fixation for immunofluorescence. Cells were visualized with DAPI staining (i and iii) or antibody staining (ii and iv) for Mcm3p-HA (B, ii and iv) or Mcm6p (C, ii and iv). (D) MCM protein localization in crm1-809 mcm3-HA (i) and crm1-11R cdc19-P1 (ii) mutant strains. Double mutant cells were incubated at the restrictive temperature (17°C) for 6 h or at 36°C for 4 h before fixation for immunofluorescence. Antibody staining is shown for Mcm6p. MCM proteins remain in the nucleus in wild-type or crm1 mutants at 17°C (our unpublished results). Bar, 10 μm.