Figure 4.

Mcm2p NLS1 is a function of NLS. Upper panel lists the fission yeast strains used for the NLS reporter assay as described in MATERIALS AND METHODS. Localization of GFP-βgal is summarized as C for cytoplasmic or N for nuclear. Lower panels show localization of NLS reporter proteins in fission yeast. Fission yeast cells expressing the GFP-βgal fusion protein were visualized by DAPI staining (i) or GFP fluorescence (ii). (A) No NLS; (B) the SV40 NLS; (C) the mutant SV40 NLS; (D) NLS1 from Mcm2p; (E) the point mutant version of NLS1 from Mcm2p-M9; and (F) NLS2 from Mcm2p. Bar, 10 μm.