Table 2.
S. pombe strains used in this study
| Strain | Genotype | Source |
|---|---|---|
| FY6 | h− leu1-32 ade6-704 ura4-294 | P. Nurse |
| FY243 | h− cdc19-P1 ade6-M210 ura4-D18 leu1-32 | Forsburg et al. (1997) |
| FY254 | h− ura4-D18 leu1-32 ade6-M210 can1-1 | Forsburg et al. (1997) |
| FY798 | h− Δcdc19::[cdc19HA::leu1+] ura4-D18 leu1-32 ade6-M210 | Snaith and Forsburg (1999) |
| FY831 | h+ pol1-1 Δcdc19::[cdc19HA leu1+] ura4-D18 leu1-32 | Derived from pol1-1D’Urso et al. (1995) |
| FY832 | h+ cdc22-M45 Δcdc19::[cdc19HA leu1+] ura4-D18 leu1-32 ade6-M210 | Forsburg et al. (1997) |
| FY835 | h+ orp1-4 Δcdc19::[cdc19HA leu1+] ura4-D18 leu1-32 ade6-M210 | Derived from orp1-4Grallert and Nurse (1996) |
| FY836 | h+ cdc21-M68 Δcdc19::[cdc19HA leu1+] ura4-D18 leu1-32 ade6-M210 | Forsburg et al. (1997) |
| FY837 | h+ cdc17-K42 Δcdc19::[cdc19HA leu1+] ura4-D18 leu1-32 ade6-M210 | Forsburg et al. (1997) |
| FY838 | h− leu1-32::leu1+/nmt/GFP-lacZ ura4-294 ade6-704 | This study |
| FY840 | h− leu1-32::leu1+/nmt/SV40 NLS-GFP-lacZ ura4-294 ade6-704 | This study |
| FY841 | h− leu1-32::leu1+/nmt/mutSV40 NLS-GFP-lacZ ura4-294 ade6-704 | This study |
| FY842 | h− leu1-32::leu1+/nmt/NLS1-GFP-lacZ ura4-294 ade6-704 | This study |
| FY843 | h− leu1-32::leu1+/nmt/NLS2-GFP-lacZ ura4-294 ade6-704 | This study |
| FY844 | h− leu1-32::leu1+/nmt/M9-GFP-lacZ ura4-294 ade6-704 | This study |
| FY894 | h− crm1-809 leu1-32 ura4-D18 ade6-M210 | Derived from crm1-809 (Nishi et al., 1994) |
| FY961 | h+ mis5-268 ura4-D18 leu1-32 ade6-M210 | Derived from Y107 (Takahashi et al., 1994) |
| FY979 | h− mis5-268 Δcdc19::[cdc19HA leu1+] leu1-32 ura4-D18 ade6-M210 | This study |
| FY1023 | h− Δmcm3::[mcm3HA leu1+] ura4-D18 leu1-32 ade6-M216 can1-1 | This study |
| FY1100 | h+ cdc19-P1 Δmcm3::[mcm3HA leu1+] ura4-D18 leu1-32 ade6-M216 | This study |
| FY1101 | h− cdc21-M68 Δmcm3::[mcm3HA leu1+] ura4-D18 leu1-32 ade6-M216 can1-1 | This study |
| FY1102 | h− crm1-809 Δmcm3::[mcm3HA leu1+] ura4-D18 leu1-32 ade6-M216 | This study |
| FY1139 | h− cdc21-M68 crm1-11R leu1-32 ura4-D18 ade6-M210 his3-D1 | This study |
| FY1152 | h− cdc19-P1 crm1-11R leu1-32 ura4-D18 ade6-M216 | This study |