Table 3.
MCM protein localization in mcm mutant strains
| A. mcm allele (phenotypea) | Temperature (°C)b | Mcm2p-HAc | Mcm4p | Mcm6p |
|---|---|---|---|---|
| cdc19-P1 (ts) | 36 | n.d. | C | C |
| cdc21-M68 (ts) | 36 | C/N | n.d. | C |
| mis5-268 (ts) | 36 | C/N | C | n.d. |
| cdc19-P1 crm1-11R (ts) | 36 | n.d. | n.d. | N |
| cdc21-M68 crm1-11R (ts) | 36 | n.d. | n.d. | N |
| B. | Mcm3p-HA | Mcm4p | Mcm6p | |
| mcm3-HA (cs) | 17 | N | C/N | C/N |
| cdc19-P1 mcm3-HA (ts) | 36 | C | C | C/N |
| cdc21-M68 mcm3-HA (cs, ts) | 36 | C | C | C |
| crm1-809 mcm3-HA (cs) | 17 | N | N | N |
MCM protein localization determined in mcm mutant strains at the restrictive temperature.
a
Temperature-sensitive phenotype: ts, thermosensitive; cs, cold sensitive.
b
Restrictive temperature at which MCM protein localization was assayed.
c
Localization was assayed by immunofluorescence analyses: N, predominantly nuclear; C, predominantly cytoplasmic; C/N, cytoplasmic and nuclear; n.d., not determined.