Figure 4. The probability to respond to the N pathway was related to the moment of N-expression rather than to the period required to implement the cellular response to Notch.

Correlation between the moment of the N^intra pulse (via a HS) after pI division (ordinate) and the time-lapse following N-activation (abscissa). The ectopic expression of Ttk in secondary anterior precursors was used to identify cell fate transformation (empty circles, both secondary precursor cells were Ttk positive). Filled circles correspond to no transformation (only one Ttk positive secondary precursor cell). Also note that the minimal time required to detect Ttk in the anterior cell was about 60 min. Note also that the transformation efficiency is higher when N^intra pulses were applied during the first hour of life (empty dots are concentrated in the bottom half) independently of the time lapse after HS. hsN^intra; neu>H2B::YFP pupae.