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. Author manuscript; available in PMC: 2008 Oct 27.
Published in final edited form as: Biochemistry. 2006 Jan 24;45(3):829–838. doi: 10.1021/bi0513709

Figure 3.

Figure 3

Mapping of 4sU+2/G36 crosslinked sites. The crosslinked sites were mapped by limited alkaline hydrolysis (Alk) and partial digestion with RNAse T1 (T1). The species consisting of 5′-32P end-labeled 4sU+2 substrate crosslinked to ribozyme 3′ strand (4sU+2*S to Rz3′ XL), and the end-labeled 4sU+2 substrate control (4sU+2*S) are paired on the left. The 5′-32P end-labeled ribozyme 3′ strand crosslinked to 4sU+2 substrate (4sU+2S to *Rz3′ XL), and end-labeled ribozyme 3′ strand control (*Rz3′) are paired on the right. Sites of T1-induced cleavages are indicated by the numbered guanosine.