. Author manuscript; available in PMC: 2008 Oct 27.

Published in final edited form as: Biochemistry. 2006 Jan 24;45(3):829–838. doi: 10.1021/bi0513709

Table 2.

Cleavage rates for +2 and +3 variants

Mutations	Ions	k_(obs) min⁻¹	k_(obs)/WT
WT (U+2, C+3)	Mg⁺⁺	0.120	1.0
C+2	Mg⁺⁺	0.002	0.017
A+2	Mg⁺⁺	0.009	0.075
G+2	Mg⁺⁺	0.003	0.025
Abasic	Mg⁺⁺	0.015	0.125
U+3	Mg⁺⁺	0.013¹	0.108
WT (U+2, C+3)	Co(NH₃)₆³⁺	0.073
C+2	Co(NH₃)₆³⁺	0.029	0.40
A+2	Co(NH₃)₆³⁺	0.023	0.32
G+2	Co(NH₃)₆³⁺	0.025	0.34
Abasic	Co(NH₃)₆³⁺	0.090	1.23
U+3	Co(NH₃)₆³⁺	0.123	1.68

Average of 2 experiments. Other experiments were performed in triplicate and values are averages.