Figure 1. Cytokine mediated regulation of CYP4F expression in primary hepatocytes.

Rat hepatocytes were treated with either vehicle or IL-1β (5ng/3mL), IL-6 (5ng/3mL), TNF-α (5ng/3mL) and IL-10 (5ng/3mL). RNA was harvested at 8hr or 24 hr post-treatment and expression of CYP4F isoforms quantitated by QRTPCR as described earlier. Each data point represents the Mean ± SEM of five independent culture plates and is expressed as percent control. * indicates P < 0.05 compared to control.