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. Author manuscript; available in PMC: 2008 Oct 27.
Published in final edited form as: Biochemistry. 2007 Jan 9;46(1):106–119. doi: 10.1021/bi061944p

Figure 5.

Figure 5

Interaction of CYP3A4(C58,C64)-CPM with ANF monitored by the changes in the fluorescence of the probe. (a) A series of emission spectra of 1.5 µM CYP3A4(C58,C64)-CPM recorded at 0, 0.4, 1.2, 2, 3, 4, 5, 8, 10, 12, 16 and 24 µM of ANF. (b) Relative fluorescence intensity versus substrate concentration. The lines show the fitting of the data sets to the Hill equation with S50 = 13 µM and n = 1.1. Experimental conditions as indicated in Figure 3.