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. Author manuscript; available in PMC: 2008 Oct 27.
Published in final edited form as: Biochemistry. 2007 Jan 9;46(1):106–119. doi: 10.1021/bi061944p

Figure 7.

Figure 7

Effect of TST on the fluorescence of CYP3A4(C58,C64)-BADAN in the presence of 25 µM ANF. (a) A series of emission spectra of CYP3A4(C58,C64)-BADAN (3 µM) in the presence of 25 µM ANF recorded at 0, 10, 20, 30, 40, 66, and 92 µM TST. (b) Changes in the relative intensity of fluorescence of CYP3A4(C58,C64)-BADAN upon addition of testosterone in the presence of 25 µM ANF. The line show the fitting of the data set to the Michaelis-Menten equation with KM = 38 µM and the maximal amplitude of fluorescence increase of 37 %. Experimental conditions as indicated in Figure 3.