. Author manuscript; available in PMC: 2008 Oct 27.

Published in final edited form as: Biochemistry. 2007 Jan 9;46(1):106–119. doi: 10.1021/bi061944p

Table 2.

Interactions of ANF with CYP3A4 modified with BADAN, CPM or mBBr monitored by the fluorescence of the probe

Protein	Parameter	Added compound

		none	1-PB, 15 µM	TST, 100 µM

CYP3A4(C58,C64)-BADAN	S₅₀, µM	18.2 ± 0.7	23.7 ± 3.5	21.4 ± 7.2
	n	1.7 ± 0.1	1.8 ± 0.3	1.6 ± 0.2
	Δ I, %^a	− 49 ± 8	− 55 ± 6	− 32 ± 12

CYP3A4(C58,C64)-CPM	S₅₀, µM	13.7 ± 4.0	n/d	n/d
	n	1.1 ± 0.25	n/d	n/d
	Δ I, %^a	−60 ± 10	n/d	n/d

CYP3A4(C58,C64)-mBBr	S₅₀, µM	12.3 ± 1.4	n/d	n/d
	n	1.1 ± 0.3	n/d	n/d
	Δ I, %^a	25 ± 16	n/d	n/d

Maximal relative change in the intensity of fluorescence of the probe.

All parameters given in the table represent the averages of two to four individual measurements. The deviations represent the confidence intervals calculated for p = 0.05.