. Author manuscript; available in PMC: 2008 Oct 27.

Published in final edited form as: Biochemistry. 2007 Jan 9;46(1):106–119. doi: 10.1021/bi061944p

Table 3.

Kinetic parameters of H₂O₂-dependent heme depletion of CYP3A4(C58,C64) labeled with BADAN, CPM, or mBBr

Protein	Substrate	k₁	k₂	F₁^a	F₁^b (fluor.)	Efficiency of FRET, %^c
CYP3A4(C58,C64)-BADAN	none	0.16 ± 0.05	0.027 ± 0.024	66 ± 16	0	79 ± 1
CYP3A4(C58,C64)-BADAN	ANF^d	0.27 ± 0.10	0.032 ± 0.010	59 ± 13	0	72 ± 6
CYP3A4(C58,C64)-CPM	none	0.38 ± 0.10	0.044 ± 0.011	60 ± 30	0	69 ± 9
CYP3A4(C58,C64)-CPM	ANF	0.29 ± 0.03	0.038 ± 0.10	66 ± 23	8 ± 2	73 ± 4
CYP3A4(C58,C64)-mBBr	none	0.25 ± 0.1	0.034 ± 0.010	47 ± 20	8 ± 3	65 ± 10
CYP3A4(C58,C64)-mBBr	ANF	0.18 ± 0.10	0.025 ± 0.010	42 ± 7	15 ± 2	65 ± 13

The fraction of the fast phase in the kinetics of heme depletion.

The fraction of the fast phase in the kinetics of H₂O₂-dependent fluorescence increase.

Apparent efficiency of FRET to the heme calculated from the amplitude of H₂O₂-dependent fluorescence increase determined by the fiiting of the kinetic curve to a biexponential equation.

50 µM.

All parameters given in the table represent averages of two to four individual measurements. The deviations represent the confidence intervals calculated for p = 0.05.