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Aggregation number, n, of α-helical bundle and cofactor-bundle complex determined by analytical ultracentrifugation. The average number of detergent molecules per protein complex were obtained as previously described (42). The empty space indicates that aggregation number has not been measured.
| n (aggregation number) in C8E5 | n in OG | ||
|---|---|---|---|
| Wavelength | 280 nm | 410 nm | 280 nm |
| Apo-AP1 | 3.6 ± 0.2 | N/A | 4.0 ± 0.1 (ndet =52±2) |
| Heme1-AP1 | 3.9 ± 0.1 | 4.0 ± 0.0 | |
| Heme2-AP1 | 3.4 ± 0.1 | 3.2 ± 0.1 | |
