TABLE 2.
METHYLATION-SPECIFIC PCR AND BISULFITE GENOMIC SEQUENCING (BGS) PRIMER SETS
| Primer Sets | |
|---|---|
| Rat M | F 5′-AGTTTTCGTCGGGTGATTTC-3′ |
| R 5′-CAAAAACTCAATTTATTTCTAACGCT-3′ | |
| Rat U | F 5′-GAGTTTTTGTTGGGTGATTTTGT-3′ |
| R 5′-AAAAACTCAATTTATTTCTAACACT-3′ | |
| Rat BGS | F 5′-AGTTTTTGGTTTTGTTTTTTATTTAGAG-3′ |
| R 5′-ACTAACCCCACCCTAATACTATTTC-3′ | |
| Human M | F 5′-TATTTTTATATTAATGCGGGATCGT-3′ |
| R 5′-ACTAACCCACCCTAATACTATTTC-3′ | |
| Human U | F 5′-TTATTTTTATATATTAATGTGGGATTGT-3′ |
| U 5′-CCACCTAAACTAAAATCTTCCACT-3′ |
Definition of abbreviations: M = methylated; U = unmethylated.