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. Author manuscript; available in PMC: 2008 Oct 28.
Published in final edited form as: Bioconjug Chem. 2008 Aug 27;19(10):1987–1994. doi: 10.1021/bc800144a

Fig. 6.

Fig. 6

Promoter-dependent enhancement of nuclear transport of pDNA by Pluronic P123 in NIH 3T3 cells. (A) Confocal microscopy images of live cells transfected (2 h) with pNFκB-luc or pAP-1-luc using Exgen 500 polyplexes and then cultured for 2 h or 3 h with or without 0.03% Pluronic P123. Signal corresponds to YOYO-1 labeled pDNA. (B) Flow cytometery of isolated nuclei of cells transfected with pAP-1-luc (as a control B1, B2) and either pNFκB-luc (B1) or gWIZLuc (B2). Cells were transfected using the same procedure as in (A) and then cultured for 3 h with or without 0.03% Pluronic P123. (B) ** p < 0.01, *** p < 0.001, n.s., not significant (n = 3).