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. Author manuscript; available in PMC: 2009 Oct 1.
Published in final edited form as: Toxicol In Vitro. 2008 Aug 15;22(7):1761–1767. doi: 10.1016/j.tiv.2008.08.003

Figure 5. Induction of BiP/GRP78 expression by selenocompounds in human lung cells.

Figure 5

Cell lysates were homogenized and 10 μg of protein was loaded onto a NuPAGE 10% Bis-Tris gel and transferred to a PVDF membrane for immunochemical analysis as described in the Methods. Cells were incubated with the selenocompounds for 24 hrs at the same concentrations indicated in figure 4. Immunochemical analysis of α-tubulin was utilized as a loading control. Immunochemical analysis of BiP/GRP78 was evaluated as an indicator of the unfolded protein response. A representative blot of triplicate experiments is shown in the upper panel and densitometry for all experiments is shown in the lower panel with MSA demonstrating a significant difference compared to the control (*, P<0.05).