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. Author manuscript; available in PMC: 2009 Sep 15.
Published in final edited form as: Free Radic Biol Med. 2008 Jun 16;45(6):847–854. doi: 10.1016/j.freeradbiomed.2008.06.008

Fig. 1. Effects of 5-HT on protein carbonylation in the right ventricle.

Fig. 1

Isolated rat hearts were subjected to retrograde perfusion on a Langendorff apparatus. After 30 min equilibration with KH buffer, the solution containing 1 μM 5-HT in KH buffer was perfused for 10 or 30 min. (A) The right ventricular wall was dissected and homogenized. Samples were derivatized with DNPH to label carbonyl moieties and subjected to SDS-PAGE and immunoblotting with the antibody which interacts with DNPH-derivatized proteins. Total protein levels were visualized by Coomassie Blue staining. Bar graphs represent means ± SEM (n = 3). (*) denotes values significantly different from each other at P < 0.05. (B) DNPH-derivatized right ventricular homogenates from perfused hearts untreated or treated with 5-HT for 10 min were subjected to isoelectric focusing followed by SDS-PAGE to obtain 2-dimensional gel electrophoresis data. After electrophoresis, slab gels were electroblotted to nitrocellulose membranes. The membrane underwent immunoblotting with the antibody, which interacts with DNPH-derivatized proteins.