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. Author manuscript; available in PMC: 2009 Sep 15.
Published in final edited form as: Free Radic Biol Med. 2008 Jun 16;45(6):847–854. doi: 10.1016/j.freeradbiomed.2008.06.008

Fig. 7. Effects of MAO-A inhibition on protein carbonylation in the right ventricle.

Fig. 7

Isolated rat hearts were subjected to retrograde perfusion on a Langendorff apparatus. After equilibration, hearts were perfused with 5-HT (1 μM) for 10 min, clorgyline (10 μM) for 30 min, or clorgyline pre-treatment for 30 min plus 5-HT for 10 min. Right ventricular wall was dissected and homogenized. Protein samples were derivatized with DNPH and subjected to SDS-PAGE and immunoblotting with the antibody, which interacts with DNPH-derivatized proteins. The bar graph represents means ± SEM (n = 3). (*) denotes values significantly different from each other at P < 0.05.