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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Mol Microbiol. 2008 Jun 28;69(3):621–632. doi: 10.1111/j.1365-2958.2008.06308.x

Figure 3.

Figure 3

FtsZ localizes to the septum independently of YycG. (A) FtsZ (red) localization was visualized immunologically in strain JH25033 grown three hours either in the presence of 1 mM IPTG (left panel) or in the absence of IPTG (right panel) to induce YycG depletion. DNA (blue) was visualized by DAPI staining. (B) Cellular protein levels of YycG and FtsZ were visualized by western blotting using whole cell protein extract derived from strain JH25033 grown three hours either in the absence (lane 1) or presence of 1 mM IPTG (lane 2) or from wild type strain JH642 (lane3) as reference; Lane M, molecular weight standard.