Figure 4.

YycG co-precipitates with FtsZ in an immunoprecipitation assay. Exponentially growing KP444 cells in the presence of IPTG (OD₅₂₅=0.3) were collected and suspended in media either supplemented with 1 mM IPTG or not. After 1 h, cultures were subjected to cross-linking and immunoprecipitation from cell extracts with purified anti-FtsZ antibodies as described in Material and Methods. FtsZ (left panel) and YycG (right panel) proteins were visualized immunologically by Western blot analysis using anti-YycG or anti-FtsZ antibody, respectively. Lanes 1 an 2 of each gel contain cross-linked sample immunoprecipitated with anti-FtsZ antibody from KP444 grown in the presence of 1 mM IPTG (lane 1) or in its absence (lane 2).