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. Author manuscript; available in PMC: 2009 Sep 1.
Published in final edited form as: J Neurochem. 2008 Jul 12;106(5):2119–2130. doi: 10.1111/j.1471-4159.2008.05564.x

Figure 2. Mapping the interacting domains on HDAC6 and tau.

Figure 2

A, Schematic diagram of C-terminal HA tagged full-length, truncated and catalytically inactive HDAC6 constructs. FL, full-length; BUZ, binder of ubiquitin zinc finger; SE14, Ser/Glu tetradecapeptide repeat domain; CD, catalytic domain; dm, double mutant. B, SE14 domain on HDAC6 is required for efficient interaction with tau. Cell lysates from HEK cells transfected with tau and different HDAC6 constructs were immunoprecipitated with HA antibody and immunoblotted as indicated. Total tau input was shown by immunoblotting cell lysates with Tau5 antibody. C, Schematic diagram of C-terminal V5 tagged full-length and truncated tau constructs. N, N-terminal region; C, C-terminal region; RD, microtubule binding repeat domain. D, Microtubule binding repeat domain on tau is necessary and sufficient for interaction with HDAC6. Cell lysates from HEK cells transfected with HDAC6-HA and different tau constructs were immunoprecipitated with HA antibody and immunoblotted as indicated. Total tau input was shown by immunoblotting cell lysates with anti-V5.