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. Author manuscript; available in PMC: 2009 Jul 8.
Published in final edited form as: Biochemistry. 2008 Jun 13;47(27):7304–7316. doi: 10.1021/bi800088t

Figure 2.

Figure 2

Spectroscopic characterization of Tlr0924 at room temperature. (a) Absorbance spectroscopy. Purified Tlr0924 exhibited peak absorbance at 434 nm (solid, PbS state). Irradiation of this peak resulted in a new peak wavelength of 538 nm (dashed, Pg). (b) CD spectroscopy. CD spectra were taken with Tlr0924 in the PbS (solid) or Pg (dashed) states. In both cases, the transition at lowest energy corresponded well to that observed in absorbance spectroscopy and exhibited negative rotation. Both states also exhibited second transitions (PbS, 336 nm; Pg, 344 nm) which exhibited positive rotation.